Pulsed-Field Gel Electrophoresis (PFGE) standardization protocol for Proteus mirabilis
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چکیده
منابع مشابه
Fingerprinting Viral Assemblages by Pulsed Field Gel Electrophoresis (PFGE)
Viruses are the most abundant microorganisms in marine and freshwater environments and perhaps the most genetically diverse (Fuhrman and Suttle, 1993). Counting viruses in aquatic samples is now a routine matter, but assessing the diversity and dynamics within complex assemblages is still a challenge. DNA-based fingerprinting approaches, which rely on amplification of rRNA gene fragments by PCR...
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PFGE is a valuable tool for assessing Listeria monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes. As DNA fragments are too large to be separated by traditional electrophoresis in an agarose gel, changes i...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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There are approximately 1.4 million Salmonella infections annually in the US. PulseNet USA detects multi-state and regional clusters and outbreaks by the use of pulsefield gel electrophoresis (PFGE) of foodborne pathogens, and assists in the investigation of potential common source outbreaks. On July 14, 2004, PA Department of Health noted a cluster of Salmonella Javiana cases in PA involving f...
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To detect Salmonella clusters, public health laboratories perform pulsed-field gel electrophoresis (PFGE) that provides a PFGE pattern, or DNA fingerprint. If the PFGE patterns of isolates from >2 persons are indistinguishable, the responsible bacteria may be related to a common source (1–3). PulseNet is a network of public health laboratories coordinated by the Centers for Disease Control and ...
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ژورنال
عنوان ژورنال: Journal of Clinical and Experimental Investigations
سال: 2013
ISSN: 1309-6621
DOI: 10.5799/ahinjs.01.2013.03.0290